Decrease in membrane fluidity and traction force induced by silica-coated magnetic nanoparticles

Abstract Background Nanoparticles are being increasingly used in biomedical applications owing to their unique physical and chemical properties small size. However, biophysical assessment evaluation of side-effects remain challenging. We addressed this issue by investigating the effects silica-coated magnetic nanoparticles containing rhodamine B isothiocyanate [MNPs@SiO 2 (RITC)] on aspects, such as membrane fluidity traction force human embryonic kidney 293 (HEK293) cells. further extended our understanding cells using a combination metabolic profiling transcriptomic network analysis. Results Overdose (1.0 ?g/µL) treatment with MNPs@SiO (RITC) induced lipid peroxidation decreased HEK293 In addition, were morphologically shrunk, aspect ratio was significantly decreased. found that each (measured micropillar) increased, thereby increasing total (RITC)-treated Due reduction elevation force, velocity cell movement also Moreover, intracellular level adenosine triphosphate (ATP) dose-dependent manner upon (RITC). To understand these changes cells, we analysed transcriptome profiles generated metabotranscriptomics network, which revealed relationships among lipids, focal adhesion, movement, related genes metabolites. Furthermore, silico prediction showed increment lipids suppression adhesion movement. Conclusion Taken together, results demonstrated overdose impairs cellular followed thus highlighting need for nanoparticle-induced side-effects.